V-700 Series UV-Visible/NIR Spectrophotometers
The series includes:
V-730 UV-Vis Spectrophotometer
Routine analysis through advanced research methods. The V-730 is an economical, general-purpose instrument with a compact design to...
V-750 UV-Vis Spectrophotometer
The V-750 is a high resolution UV-visible double-beam spectrophotometer with single monochromator, variable spectral bandwidth and a...
V-760 UV-Vis Spectrophotometer
The V-760 is a high resolution UV-visible double-beam spectrophotometer with double monochromator for exceptional stray light and...
V-770 UV-Vis Spectrophotometer
The V-770 UV-Visible-Near Infrared Spectrophotometer is a unique optical design incorporating a dual-grating, dual-detector design...
V-780 UV-Vis Spectrophotometer
The V-780 UV-Visible-Near Infrared Spectrophotometer is a unique optical design incorporating a dual-grating, dual-detector design...
Features
Compact Design
The compact optical design of the V-700 Series reduces space requirements in the modern laboratory.
Excellent optical performance
Reduced stray light enables highly precise measurements over a wide photometric range.
High speed scanning
High throughput optics and fast detectors provide high-speed scanning without wavelength tracking errors.
Dark correction
Stray light reduction features enable highly-accurate measurements of high-absorbance samples.
Monochromator step scanning
Step scan function provides reliable peak assignment of samples with sharp or narrow absorption peaks.
User-friendly graphical interfaces
New iRM intelligent remote module, Spectra Manager II and Spectra Manager CFR control and analysis systems
IQ Accessory
Automatic accessory recognition.
IQ Start
A registered software program can be activated with a simple push of the 'Start Button'.
Full range of Accessories
Over 50 sampling accessories for gas, liquid and solid samples are available for specific sampling applications.
Accurate and reliable
A USP, EP and JP compliant instrument validation is provided. IQ/OQ support is available as an option.
Wide wavelength range from UV to NIR
High energy throughput provides excellent signal-to-noise ratio for the entire UV to NIR spectral range.
V-700 Series Specifications
V-730 UV-Vis Spectrophotometer Specifications
| Optical System | Rowland off-circle arrangement Single monochromator Double beam type |
| Light source | Halogen lamp, Deuterium lamp |
| Wavelength range | 190 to 1100 nm |
| Wavelength accuracy | +/-0.2 nm (at 656.1 nm) |
| Wavelength repeatability | +/-0.1 nm |
| Spectral bandwidth (SBW) | 1 nm |
| Stray light | 1 % (198 nm KCL 12 g/L aqueous solution) 0.02 % (220 nm NaI 10 g/L aqueous solution) 0.02 % (340 nm NaNO2 50 g/L aqueous solution) 0.02 % (370 nm NaNO2 50 g/L aqueous solution) SBW: 1 nm |
| Photometric range | -3~3 Abs |
| Photometric accuracy | +/-0.0015 Abs (0 to 0.5 Abs) +/-0.0025 Abs (0.5 to 1 Abs) +/-0.3 %T Tested with NIST SRM 930D |
| Photometric repeatability | +/-0.0005 Abs (0 to 0.5 Abs) +/-0.0005 Abs (0.5 to 1 Abs) Tested with NIST SRM 930D |
| Scanning speed | 10-8000 nm/min |
| Slew speed | 24,000 nm/min |
| RMS noise | 0.00004 Abs (0 Abs, wavelength: 500 nm, measurement time: 60 sec, SBW: 1 nm) |
| Baseline stability | 0.0004 Abs/hour (Value obtained more than one hour after turning on the source, when the room temperature is stabilized, wavelength: 250 nm, response: slow) |
| Baseline flatness | +/-0.0005 Abs (200 - 1000 nm) |
| Detector | Silicon photodiode |
| Standard facilities | IQ accessories, Start button, Analog output |
| Standard program | Abs/%T meter, Quantitative analysis, Spectrum measurement, Time course measurement, Fixed wavelength measurement, Validation, Daily maintenance, Macro command (only for iRM), Two wavelengths time course measurement (only for PC) |
| Dimensions and weight | 486(W) x 441(D) x 216(H) mm, 15 kg |
| Power requirements | 120 VA |
| Installation requirements | Room temperature: 15-30 Celsius, humidity: below 85% |
V-750 UV-Vis Spectrophotometer Specifications
| Optical System | Czerny-Turner mount Single monochromator Fully symmetrical double beam type |
| Light Source | Halogen lamp, Deuterium lamp |
| Wavelength range | 190 to 900 nm |
| Wavelength accuracy | +/-0.2 nm (at 656.1 nm) |
| Wavelength repeatability | +/-0.05 nm |
| Spectral bandwidth (SBW) | 0.1, 0.2, 0.5, 1, 2, 5, 10 nm L2, L5, L10 nm (low stray light mode) M1, M2 nm (micro cell mode) |
| Stray light | 1 % (198 nm KCL 12 g/L aqueous solution) 0.005 % (220 nm NaI 10 g/L aqueous solution) 0.005 % (340 nm NaNO2 50 g/L aqueous solution) 0.005 % (370 nm NaNO2 50 g/L aqueous solution) SBW: L2 nm |
| Photometric range | -4~4 Abs |
| Photometric accuracy | +/-0.0015 Abs (0 to 0.5 Abs) +/-0.0025 Abs (0.5 to 1 Abs) +/-0.3 %T Tested with NIST SRM 930D |
| Photometric repeatability | +/-0.0005 Abs (0 to 0.5 Abs) +/-0.0005 Abs (0.5 to 1 Abs) Tested with NIST SRM 930D |
| Scanning speed | 10~4000 nm/min (8000 nm/min in preview mode) |
| Slew speed | 12,000 nm/min |
| RMS noise | 0.00003 Abs (0 Abs, wavelength: 500 nm, measurement time: 60 sec, SBW: 2 nm) |
| Baseline stability | 0.0003 Abs/hour (Value obtained more than two hours after turning on the source, when the room temperature is stabilized, wavelength: 250 nm, response: slow and SBW: 2 nm) |
| Baseline flatness | +/-0.0002 Abs (200 - 850 nm) |
| Detector | Photomultiplier tube |
| Standard facilities | IQ accessories, Start button, Analog output |
| Standard program | Abs/%T meter, Quantitative analysis, Spectrum measurement, Time course measurement, Fixed wavelength measurement, Validation, Daily maintenance, Macro command (only for iRM), Two wavelengths time course measurement (only for PC) |
| Dimensions and weight | 460(W) x 602(D) x 268(H) mm, 27 kg |
| Power requirements | 150 VA |
| Installation requirements | Room temperature: 15-30 Celsius, humidity: below 85% |
V-760 UV-Vis Spectrophotometer Specifications
| Optical System | Czerny-Turner mount Double monochromator Fully symmetrical double beam type |
| Light Source | Halogen lamp, Deuterium lamp |
| Wavelength range | 187 to 900 nm |
| Wavelength accuracy | +/-0.1 nm (at 656.1 nm) |
| Wavelength repeatability | +/-0.05 nm |
| Spectral bandwidth (SBW) | 0.1, 0.2, 0.5, 1, 5, 2, 10 nm L2, L5, L10 nm (low stray light mode) M1, M2 nm (micro cell mode) |
| Stray light | 1 % (198 nm KCL 12 g/L aqueous solution) 0.00008 % (220 nm NaI 10 g/L aqueous solution) 0.00008 % (340 nm NaNO2 50 g/L aqueous solution) 0.00008 % (370 nm NaNO2 50 g/L aqueous solution) SBW: L2 nm |
| Photometric range | -4~6 Abs |
| Photometric accuracy | +/-0.0015 Abs (0 to 0.5 Abs) +/-0.0025 Abs (0.5 to 1 Abs) +/-0.3 %T Tested with NIST SRM 930D |
| Photometric repeatability | +/-0.0005 Abs (0 to 0.5 Abs) +/-0.0005 Abs (0.5 to 1 Abs) Tested with NIST SRM 930D |
| Scanning speed | 10~4000 nm/min (8000 nm/min in preview mode) |
| Slew speed | 12,000 nm/min |
| RMS noise | 0.00003 Abs (0 Abs, wavelength: 500 nm, measurement time: 60 sec, SBW: 2 nm) |
| Baseline stability | 0.0003 Abs/hour (Value obtained more than two hours after turning on the source, when the room temperature is stabilized, wavelength: 250 nm, response: slow and SBW: 2 nm) |
| Baseline flatness | +/-0.0003 Abs (200 - 800 nm) |
| Detector | Photomultiplier tube |
| Standard facilities | IQ accessories, Start button, Analog output |
| Standard program | Abs/%T meter, Quantitative analysis, Spectrum measurement, Time course measurement, Fixed wavelength measurement, Validation, Daily maintenance, Macro command (only for iRM), Two wavelengths time course measurement (only for PC) |
| Dimensions and Weight | 460(W) x 602(D) x 268(H) mm, 29 kg |
| Power requirements | 150 VA |
| Installation requirements | Room temperature: 15-30 Celsius, humidity: below 85% |
V-770 UV-Visible-Near Infrared Spectrophotometer Specifications
| Optical System | Czerny-Turner mount Single monochromator Fully symmetrical double beam type |
| Light Source | Halogen lamp, Deuterium lamp |
| Wavelength range | 190 to 2700 nm (3200 nm, option) |
| Wavelength accuracy | +/-0.3 nm (at 656.1 nm) +/-1.5 nm (at 1312.2 nm) |
| Wavelength repeatability | +/-0.05 nm (UV-Vis), +/-0.2 nm (NIR) |
| Spectral bandwidth (SBW) | UV-Vis: 0.1, 0.2, 0.5, 1, 2, 5, 10 nm L2, L5, L10 nm (low stray light mode) M1, M2 nm (micro cell mode) NIR: 0.4, 0.8, 1, 2, 4, 8, 20, 40 L8, L20, L40 nm (low stray light mode) M4, M8 nm (micro cell mode) |
| Stray light | 1 % (198 nm KCL 12 g/L aqueous solution) 0.005 % (220 nm NaI 10 g/L aqueous solution) 0.005 % (340 nm NaNO2 50 g/L aqueous solution) 0.005 % (370 nm NaNO2 50 g/L aqueous solution) SBW: L2 nm 0.04 % (1420 nm: H2O) |
| Photometric range | UV-Vis: -4~4 Abs NIR: -3~3 Abs |
| Photometric accuracy | +/-0.0015 Abs (0 to 0.5 Abs) +/-0.0025 Abs (0.5 to 1 Abs) +/-0.3 %T Tested with NIST SRM 930D |
| Photometric repeatability | +/-0.0005 Abs (0 to 0.5 Abs) +/-0.0005 Abs (0.5 to 1 Abs) Tested with NIST SRM 930D |
| Scanning speed | 10~4000 nm/min (8000 nm/min in preview mode) |
| Slew speed | UV-Vis: 12,000 nm/min NIR: 48,000 nm/min |
| RMS noise | 0.00003 Abs (0 Abs, wavelength: 500 nm, measurement time: 60 sec, SBW :2 nm) |
| Baseline stability | 0.0003 Abs/hour (Value obtained more than two hours after turning on the source, when the room temperature is stabilized, wavelength: 250 nm, response: slow and SBW: 2 nm) |
| Baseline flatness | +/-0.0002 Abs (200 - 2500 nm) |
| Detector | Photomultiplier tube, Peltier cooled PbS |
| Standard facilities | IQ accessories, Start button, Analog output |
| Standard program | Abs/%T Meter, Quantitative Analysis, Spectrum Measurement, Time Course Measurement, Fixed-Wavelength Measurement, Validation, Daily Maintenance, Macro command (only for iRM), Dual Wavelength Time Course Measurement (only for PC |
| Dimensions and weight | 460(W) x 602(D) x 268(H) mm, 29 kg |
| Power requirements | 150 VA |
| Installation requirements | Room temperature: 15-30 Celsius, humidity: below 85% |
V-780 UV-Vis Spectrophotometer Specifications
| Optical System | Czerny-Turner mount Single monochromator Fully symmetrical double beam type |
| Light Source | Halogen lamp, Deuterium lamp |
| Wavelength range | 190 to 1600 nm |
| Wavelength accuracy | +/-0.3 nm (at 656.1 nm) +/-1.0 nm (at 1312.2 nm) |
| Wavelength repeatability | +/-0.05 nm (UV-Vis), +/-0.1 nm (NIR) |
| Spectral bandwidth (SBW) | UV-Vis: 0.1, 0.2, 0.5, 1, 2, 5, 10 nm L2, L5, L10 nm (low stray light mode) M1, M2 nm (micro cell mode) NIR: 0.2, 0.4, 0.5, 1, 2, 4, 10, 20, L4, L10, L20 nm (low stray light mode) M2, M4 nm (micro cell mode) |
| Stray light | 1 % (198 nm KCL 12 g/L aqueous solution) 0.005 % (220 nm NaI 10 g/L aqueous solution 0.005 % (340 nm NaNO2 50 g/L aqueous solution) 0.005 % (370 nm NaNO2 50 g/L aqueous solution) SBW: L2nm 0.04 % (1420 nm: H2O) |
| Photometric range | UV-Vis: -4~4 Abs NIR: -3~3 Abs |
| Photometric accuracy | +/-0.0015 Abs (0 to 0.5 Abs) +/-0.0025 Abs (0.5 to 1 Abs) +/-0.3 %T Tested with NIST SRM 930D |
| Photometric repeatability | +/-0.0005 Abs (0 to 0.5 Abs) +/-0.0005 Abs (0.5 to 1 Abs) Tested with NIST SRM 930D |
| Scanning speed | 10~4000 nm/min (8000 nm/min in preview mode) |
| Slew speed | UV-Vis: 12000 nm/min NIR: 24000 nm/min |
| RMS noise | 0.00003 Abs (0 Abs, wavelength: 500 nm, measurement time: 60 sec, SBW:2 nm) |
| Baseline stability | 0.0003 Abs/hour 0.04 % (220 nm NaI 10 g/L aqueous solution) 0.02 % (340 nm NaNO2 50 g/L aqueous solution) 0.02 % (370 nm NaNO2 50 g/L aqueous solution) (10 mm cell) |
| Baseline Stability | ±0.0004 Abs/hour (Value obtained more than two hours after turning on the light source, when the room temperature is stabilized, wavelength: 250 nm, response: slow and SBW: 2nm.) |
| Baseline flatness | +/-0.0002 Abs (200 - 1600 nm) |
| Detector | Photomultiplier tube, Peltier cooled InGaAs photodiode |
| Standard facilities | IQ accessories, Start button, Analog output |
| Standard program | Abs/%T Meter, Quantitative Analysis, Spectrum Measurement, Time Course Measurement, Fixed-Wavelength Measurement, Validation, Daily Maintenance, Macro command (only for iRM), Dual Wavelength Time Course Measurement (only for PC |
| Dimensions and weight | 460(W) x 602(D) x 268(H) mm, 29 kg |
| Power requirements | 150 VA |
| Installation requirements | Room temperature: 15-30 Celsius, humidity: below 85% |
Accessories
Integrating spheres for the V-700 UV-Vis/NIR spectrophotometers
Integrating spheres are designed to measure either the diffuse transmittance or diffuse reflectance spectra of a liquid, solid or powder sample.
ISV-922/ISN-923/ISN-901i/ILV-924/ILN-925/ILN-902i/PIV-756/PIN-757/PIN-903i/IJV-726/IJN-727/IJN-904i/HISV-728/HISN-729 integrating sphere accessories
For standard measurements, UV-Vis or UV-Vis/NIR spectrophotometers measure the transmittance of a homogeneous, transparent liquid or solid sample. However, when a suspended liquid sample or opaque solid or powder sample is measured, the light incident upon the sample is diffusely transmitted or reflected and only a small portion of the scattered light reaches the detector, resulting in an inaccurate measurement. The integrating sphere accessory acquires most of the light diffusely transmitted or diffusely reflected from the sample into the integrating sphere and introduces it to the detector(s), providing a more representative measurement of the diffuse sample. JASCO integrating spheres have been designed for a variety of application and sampling requirements.
ISV-922 60 mm Integrating sphere for V-750/760
ISN-923 60 mm Integrating sphere for V-770
ISN-901i 60 mm Integrating sphere for V-780
The Model ISV-922/ISN-923/ISN-901i accessories are 60 mm integrating spheres with a smaller footprint to fit directly into the V-700 instrument sample compartment. These integrating spheres have a smaller sphere area, resulting in an increased signal-to-noise ratio for sample measurements at smaller bandwidth settings. All integrating spheres include a Spectralon reference tile, used for baseline correction of the integrating sphere. In addition, a specular reflectance exclusion sample holder is included as standard as well as a modified sample holder which will include the specular reflectance data with the collected spectrum. The 60 mm spheres have been designed with several different configurations to provide simple sampling methods for powders, solids and other scattering samples.
FCH-002 Fluorescence cut-off filter
A fluorescence cut-off filter permits accurate measurement of the transmittance/reflectance of a sample that also emits fluorescence (commonly, the 450 nm to 650 nm range with excitation near 300 nm). The optional filter is installed inside the sphere, before the internal sphere PMT detector. (Must be ordered with the integrating sphere, installed during manufacture.)
PSH-001 Powder Sample Holder
This optional accessory is used to measure the reflectance of a powdered sample.
Model RLH-603 Reference-Side Rectangular Cell Holder
This optional cell holder is used to install a rectangular cell on the reference side of the integrating sphere
(used as a reference for diffuse transmittance samples).
SSH-506 Solid Sample Holder (specific to 60 mm integrating spheres)
This sample holder is used to measure the transmittance of a solid sample.
Sample size:
Minimum 20 (H) x 20 (W) x 0.5 (D) mm
Maximum 70 (H) x 40 (W) x 35 (D) mm
60 mm Integrating Sphere specifications
|
Wavelength range |
200 to 900 nm (ISV-922) |
|
Spectral bandwidth |
UV 5 nm |
|
Sample cell (Transmittance measurement) |
Light path length 5, 10, 20, 30, 50 mm rectangular cell (sample side) |
|
Sample size (Reflectance measurement) |
Minimum 20 (Height) x 20 (Width) x 0.5 (Thickness) mm |
|
Beam |
Approx. 7 (Height) x 4 (Width) mm at the position of the transmittance sample Approx. 8 (Height) x 9 (Width) mm at the position of the reflectance sample |
|
Inside diameter of integrating sphere |
60 mm |
|
Interior of integrating sphere |
Coated with barium sulfate |
|
Detector |
Photomultiplier tube (ISV-922/ISN-923/ISN-901i) |
ILV-924 150 mm Integrating sphere for V-750/760
ILN-925 150 mm Integrating sphere for V-770
ILN-902i 150 mm Integrating sphere for V-780
The Model ILV-924/ILN-925/ILN-902i accessories are 150 mm integrating spheres with a larger sampling area and fixtures for larger samples, with features for diffuse transmittance and diffuse reflectance measurements. The 150 mm spheres include an integrated light trap for exclusion of specular reflectance components from the sample. Spectralon reference tiles are included for baseline correction of the spectral data collected using the sphere. The larger sphere diameter corresponds to many international standards that require the larger sphere area, as compared to the 60 mm integrating spheres. Although the 150 mm sphere may have slightly lower signal-to-noise performance, the larger area vs. the ‘cutouts’ for the source entrances and sample area ports provides a greater integration capability for sample reflectance experiments.
Portable Integrating Sphere Specifications
|
Wavelength range |
200 to 900 nm (Model ILV-924) |
|
Spectral bandwidth |
UV 5 nm |
|
Sample cell (Transmittance measurement) |
Light path length 5, 10, 20, 30, 50 mm rectangular cell (sample side) |
|
Sample size (Reflectance measurement) |
Minimum 20 (Height) x 20 (Width) x 0.5 (Thickness) mm |
|
Beam |
Approx. 9 (Height) x 5.5 (Width) mm at the position of the Approx. 13 (Height) x 6 (Width) mm at the position of the reflectance |
|
Inside diameter of integrating sphere |
150 mm |
|
Interior of integrating sphere |
Coated with barium sulfate |
|
Detector |
Photomultiplier tube (Model ILV-924/ILN-925/ILN-902i) |
SSH-507 Solid Sample Holder (specific to 150 mm integrating spheres)
This sample holder is used to measure the transmittance of a solid sample.
Sample size:
Minimum 20 (H) x 20 (W) x 0.5 (D) mm
Maximum 70 (H) x 40 (W) x 35 (D) mm
PIV-756 60 mm Integrating sphere for V-750/760
PIN-757 60 mm Integrating sphere for V-770
PIN-903i 60 mm Integrating sphere for V-780
Integrating spheres for horizontal sampling, models PIV-756/PIN-757/PIN-903i, can mount the sample horizontally in a cup below the integrating sphere, ideal for powder samples. This integrating sphere can also be modified to provide measurements of smaller sample sizes by using a lens above the sphere, focusing the source energy onto the sample below the sphere. Diffuse transmittance measurements are performed by placing the sample on the upper surface of the integrating sphere.
IJV-726 60 mm Integrating sphere for V-750/760
IJN-727 60 mm Integrating sphere for V-770
IJN-904i 60 mm Integrating sphere for V-780
Models IJV-726/IJN-727/IJN-904i are dedicated integrating spheres to measure small samples such as gemstones. The accessory is based on a standard 60 mm integrating sphere with a ‘mask’ to provide a smaller illumination area for reflectance samples. A number of small fixtures are provided for mounting of gemstones, pearls and mounted stones within rings and other jewelry for analysis purposes.
HISV-728 60 mm Portable integrating sphere for V-750/760
HISN-729 60 mm Portable integrating sphere for V-770/780
This integrating sphere using an optical fiber can measure the diffuse reflection of various solid samples, such as pastes, clothing materials and food products.
Portable Integrating Sphere Specifications
|
Wavelength range |
250 to 800 nm (Model HISV-728) |
|
Spectral bandwidth |
UV 10 nm |
|
Sample size |
Minimum 30 (Height) x 30 (Width) |
|
Inside diameter of integrating sphere |
60 mm |
|
Interior of integrating sphere |
Coated with barium sulfate |
|
Detector |
Accessories
Ambient Accessories for V-700 Series UV-visible and Near infrared NIR spectrophotometers
UCB-710 Rectangular cell Holder with cuvette height adjustment
- Suitable for cuvettes sizes down to 2 mm width x variable path length up to 10 mm (minimum sample volume of 240 µL)
- 1.5 mm (W) x 3 mm (H) mask included as standard
LSE-701 Single position long path length cell holder
LSE-701 single position long-path cell holder for sample measurements using long light path rectangular cells (maximum: 100 mm). Typical pathlengths include 10 mm, 20 mm, 50 mm or 100 mm. A cell of the same pathlength can also be placed on the reference side.
FSE-702 manual 4-position long path length cell changer
FSE-702 manual 4-position Long-Path Cell Holder for sample measurements using long path rectangular cells (maximum: 100 mm). Typical path lengths include 10 mm, 20 mm, 50 mm or 100 mm. A cell of the same pathlength can also be used for the reference side.
SSE-704 6-position manual cell holder
SSE-704 6-position manual cell holder for up to 6 sample cell positions. The cell changer is manually operated and can be used with cell sizes of 5 mm, 10 mm or 20 mm. A cell of the same pathlength can also be used for the reference side.
CYH-708 Cylindrical cell holder
CYH-708 cylindrical cell holder for sample measurement using cylindrical cells (Diameter: 22 or 30 mm; maximum path length: 100 mm)
NCP-705 specifications
|
Sample movement |
Linear |
|
Cell type |
10-mm rectangular cell, Micro Cell (min. sample width 2 mm) |
|
Reference cell holder |
Single-cell holder |
|
Dimensions |
130 (W) x 255 (D) x 123 (H) mm |
|
Weight |
Accessories
Absolute reflectance measurement for V-700 Series UV-visible and Near infrared NIR spectrophotometers
The absolute reflectance measurement system for the V-700 series UV-visible-NIR spectrophotometers is used to perform the measurement of the spectral properties, film thickness, angle variation or other characteristics of solid samples such as semiconductors, thin films and various optical elements.
ARV-913/ARN-914/ARN-915i/ARSV-916/ARSN-917/ARSN-918i/ARMV-919/ARMN-920/ARMN-921i
The absolute reflectance measurement accessories are designed to measure the absolute reflectance of a specular reflecting sample (metals or other materials) while varying the incidence angle (all models). The ARSV-916, ARSN-917, ARSN-918i, ARMV-919, ARMN-920 and ARMN-921i accessories also permit the transmittance measurement of a sample (without diffuse transmittance), while varying the incidence angle. Absolute reflectance and transmittance are measured by rotating the sample stage to determine the angle of the light incident to the sample and setting the detector in the path of the reflected light or the transmitted light through the sample.
Diffuse reflectance measurements can be obtained by mounting the sample at the rear of the integrating sphere with an incidence angle of 0º. Diffuse transmittance measurements can also be obtained by mounting the sample at the entrance of the integrating sphere with the sample holder at an incidence angle of 0º.
Effect of polarization when measuring absolute reflectance
Light from a spectrophotometer that employs a grating is always polarized. The ratio of the intensities of the S and P polarized light varies according to the wavelength and also differs from one grating to another for the same wavelength. The greater the angle of incidence of the beam on the sample, the greater the difference in the intensity of the S and P polarized reflected light making measurement inaccurate if the absolute reflectance is measured at a high angle of incidence. In this case, a polarizer can be set to 45 degrees so that the incident light can be considered non-polarized.
Range of Absolute Reflectance Measurement Accessories
Manual Measurement
ARV-913 Synchronous Absolute reflectance measurement accessory (UV-Vis). For use with V-750 and V-760
ARN-914 Synchronous Absolute reflectance measurement accessory (UV-Vis/NIR). For use with V-770 only
ARN-915i Synchronous Absolute reflectance measurement accessory (UV-Vis/NIR). For use with V-780 only
Reflectance measurement only
The incidence and collection angles are maintained with the same ‘synchronous’ angle by simultaneously rotating the sample stage and integrating sphere.
The ARV-913, ARN-914 and ARN-715i measure the absolute reflectance of a specular reflecting sample (metals, etc.) or the relative reflectance of a diffusely reflecting sample. It also permits the measurement of transmittance by using an optional solid sample holder for transmittance measurements. This attachment includes an integrating sphere with integrated detector(s). The angle of incidence of the sample is changed by moving the integrating sphere (in the measurement of absolute reflectance). The movable angular range of the integrating sphere is 5°to 90°(angle of incidence). The relative reflectance is measured by mounting the sample on the rear of the integrating sphere.
ARSV-916 Asynchronous Absolute reflectance measurement accessory (UV-Vis) For use with V-750 and V-760
ARSN-917 Asynchronous Absolute reflectance measurement accessory (UV-Vis/NIR) For use with V-770
ARSN-918i Asynchronous Absolute reflectance measurement accessory (UV-Vis/NIR) For use with V-780
Reflectance and transmittance measurement
The incidence and collection angles can be set independently in an asynchronous mode.
The ARSV-916, ARSN-917 and ARSN-918i manual absolute reflectance measurement accessories measure the absolute reflectance of a reflective sample or transmittance of a clear sample that does not diffuse light. The detector(s) is(are) equipped with an integrating sphere and also permits measurement of the relative reflectance and transmittance of samples that diffuse light (the sample holder for measurement of relative reflectance is standard, the sample holder for transmittance measurement is optional). Absolute reflectance and transmittance are measured by rotating the sample stage to determine the angle of the light incident upon the sample and setting the detector in the path of the reflected or transmitted light. The relative reflectance is measured with light normally incident to the sample, which is set behind the integrating sphere.
Automated measurement system
ARMV-919 Automated absolute reflectance measurement accessory (UV/VIS) For use with V-750 and V-760
ARMN-920 Automated absolute reflectance measurement accessory (UV/VIS/NIR) For use with V-770
ARMN-921i Automated absolute reflectance measurement accessory (UV/VIS/NIR) For use with V-780
Reflectance and transmittance measurement
The incidence and collection angles can be set independently in asynchronous mode.
The ARMV-919, ARMN-920 and ARMN-921i absolute reflectance measurement systems automate the measurement of spectral properties, including film thickness, angle variation or other characteristics of solid samples such as semiconductors, thin films and various optical elements. The incidence and collection angles can be set in a synchronous mode, simultaneously rotating the sample stage and integrating sphere. Alternatively, the incidence and collection angles can be set independently in asynchronous mode. The polarization properties of a sample can also be examined using P or S polarization or by setting the desired polarization angles.
Absolute Reflectance optical layout
Absolute Reflectance Measurement Accessories
Optional accessories
SSH-508 Solid Sample Holder
This attachment is used to measure the transmittance of a sample that diffuses light.
Sample size :
Min 30 mm (H) x 30 mm (W) x 0.5 mm (D)
Max 70 mm (H) x 80 mm (W) x 10 mm (D)
ARG-476/GPH-506 Polarizers
The light from a grating type spectrophotometer is always polarized. The ratio of the intensities of the S and P polarized light varies according to the wavelength and also differs from one grating to another for the same wavelength. The greater the angle of incidence of the beam on the sample, the greater the difference in the intensity of the S and P polarized reflected light, making measurements inaccurate if the absolute reflectance is measured at a high angle of incidence. In this case, a polarizer is required to accurately measure the absolute reflectance when the angle of incidence is 30° or greater.
Wavelength range:
250-750 nm(Model ARV-913)
250-2000 nm(Model ARN-914)
250-1600 nm(Model ARN-915i)
Rotation of plane of polarization:
0°(linearly polarized light in vertical
direction) to 90°(linearly polarized light
in horizontal direction)
Optional accessories continued
Wide incident angle sample holder (up to 85º)
6708-H163A for manual type
6708-H460A for automated type
PDU-755 Phase difference measurement unit (Polarizer with measurement software)
Accessories
Specular Reflectance Accessories
SLM-907 Specular reflectance accessory for V-700
The SLM-907 specular reflectance accessory measures the relative reflectance of a sample in comparison with the reflected light from an aluminum-deposited plane mirror as a reference. This accessory is used for the reflectance measurement of sample types such as: metal-deposited films, metal plating, and other thin films. Film thickness can be calculated using the optional film thickness analysis program.
Specular Reflectance Accessory Specifications
Wavelength range
250 to 1000 nm (V-730 UV/VIS spectrophotometer)
200 to 900 nm (V-750/760 UV/VIS spectrophotometer)
200 to 2700 nm (V-770 UV/VIS/NIR spectrophotometer)
200 to 1600 nm (V-780 UV-Vis/NIR spectrophotometer)
Sample size
Sample
Minimum 10 x 10 mm
Maximum 100 x 120 mm
Reference
Minimum 10 x 10 mm
Maximum 20 x 20 mm
Beam port diameter
7 mm (4 mm, 2 mm as option)
Angle of Incidence
Approx. 5°
Reflection reference
Aluminum-deposited plane mirror (Standard mirror)
Optional accessories:
- Polarizer (GPH-506)
- Application program (Film thickness calculation program, etc.)
- Sample stage with 2-mm-dia. port
- Sample stage with 4-mm-dia. port
MSK-001 Sample stage with 2-mm-dia. port
MSK-002 Sample stage with 4-mm-dia. port
MSK-001 Sample stage with 2-mm-dia.port
MSK-002 Sample stage with 4-mm-dia.port
Diameter of port:
Sample Size:
2mm
Minimum 3x3 mm
Maximum 50x50 mm
Diameter of port:
Sample size:
4mm
Minimum 5x5 mm
Maximum 50x50 mm
SLM-908 Specular reflectance accessory for V-750/760/770/780 (for 6-inch Silicon Wafers)
Accessories
STR-773 Water thermostatted cell holder with stirrer
The STR-773 Water thermostatted cell holder with stirrer is used with a temperature controlled circulating water bath (not supplied) to maintain a constant sample and reference cell temperature.
The STR-773 accepts standard 10 mm path length cells. A magnetic stirrer is included to maintain temperature equilibrium in both cell positions.
Specifications
NCP-706 specifications
|
Sample movement |
Linear |
|
Cell type |
10-mm rectangular cell, Micro Cell (min. sample width 2 mm) |
|
Thermostatic method |
Circulation of constant temperature water |
|
Operating temperature |
Room temperature plus 10° -90°C |
|
Reference cell holder |
Constant temperature single-cell holder |
|
Dimensions |
130 (W) x 255 (D) x 123 (H) mm |
|
Weight |
2.5 kg |
NCP-705 6-position non-thermostatted automatic cell changer NCP-706 Water thermostatted 6-position automatic cell changer
The NCP-705 and NCP-706 6-position automatic cell changers for up to 6 sample positions. The sample position can be selected using software control and can be used with cell path lengths up to 10 mm.
A single cell is used for the reference position for both the NCP-705/706 automatic six cell changers.
The NCP-706 includes temperature control using a water-thermostatted circulator.
CSP-909 Sample Holder Lid with Syringe Port for V-700 models
The optional CSP-909 lid is used for adding a reagent solution with a syringe without having to open the sample chamber when initiating fast reactions. The syringe port lid can be used together with the STR-773, ETHS-760, ETCS-761 or ETCS-762 cell holders.
HMC-711 Water Thermostatted Micro Cell Holder (100 µL, 200 µL)
The HMC-711 Water Thermostatted Micro Cell Holder is used with a temperature controlled circulating water bath (not supplied) to maintain a constant sample temperature. The cell holder accepts standard 10 mm path length cells with a minimum path width of 2 or 4 mm.
Specifications
5 or 10 mm path length square micro cell
Optional cells
| Item | P/N | Specification |
| Rectangular micro quartz cell | 1103-0025 | 5 mm path, 2 mm width |
| Rectangular micro quartz cell | 1103-0030 | 10 mm path, 4 mm width |
| Rectangular micro quartz cell | 1103-0026 | 10 mm path, 2 mm width |
| Rectangular micro quartz black cell | 1103-0031 | 10 mm path, 4 mm width |
| Rectangular micro quartz black cell | 1103-0032 | 10 mm path, 2 mm width |
1103-0026
10 mm path, 2 mm width
100 µL minimum volume
1103-0032
10 mm path, 2 mm width
100 µL minimum volume
MHT-745 Manual 4-position water thermostatted turret cell holder
The MHT-745 is used with a temperature controlled circulating water bath. The 4-position turret cell holder can be rotated manually for measurement of up to four samples. Requires four 10x10 mm rectangular cells (not included).
Specifications
| Thermostatted cells | 10-mm rectangular cell Sample-side cell holder: 4 Reference side cell holder: 1 |
| Operating temperature | Room temperature plus 10 to 90°C |
| Circulating water inlet | Uni-tube (⌽7 x ⌽5 mm) |
EHCS-760 Peltier thermostatted single position cell holder (air-cooled)
This Peltier cell holder precisely maintains the specified sample temperature. The cell holder includes a stirrer for uniform cell temperature. The EHC-760 cell holder is an air-cooled accessory for temperature control without using a water circulator.
OPS-512 Optional temperature sensor for EHCS-760
OPS-512 OP sensor for direct temperature measurement in the sample cell for both the ETC-760 or EHC-761.
Specifications
| Thermostatted Cell | 10-mm rectangular cell (sample side) |
| Temperature control system | Heating/cooling system using Peltier effect |
| Heat radiating system | Air cooled |
| Stirring system | Magnetic stirrer (variable speed) |
| Temperature setting range | 5 to 70°C |
| Temperature control range | 10 to 60°C (for room temperature = 20°C) |
| Temperature control accuracy | ± 0.1°C |
| Temperature accuracy | (Difference between displayed temperature and actual cell temperature) 20°C to 40°C ± 0.5°C ± 1°C when set temperature is outside the above range |
| Temperature setting difference | (Difference between set temperature and displayed temperature) 20°C to 40°C ±0.3°C ± 0.5°C when set temperature is outside the above range |
PSC-763 Automatic 6-position Peltier cell changer (air-cooled)
Model PSC-761 multi-cell accessory for simultaneous temperature controlled measurement in up to 6 sample cells. This cell holder uses air-cooled Peltier effect to control sample temperature with high accuracy. Includes a variable speed stirrer for thermal equilibrium for all sample cells.
OPS-513 Optional temperature sensor for PSC-763
OPS-513 OP sensor for direct temperature measurement in the sample cell.
Specifications
| Thermostatted Cell | Up to six 10 mm rectangular sample cells (ambient reference cell) |
| Temperature control system | Heating/cooling system utilizing Peltier effect |
| Heat radiating system | Forced air cooling |
| Stirring system | Magnetic stirrer (variable speed) |
| Temperature setting range | 10 to 70°C |
| Temperature control range | 15 to 60°C (For room temperature = 20°C) |
| Temperature control accuracy | ±0.1°C |
| Temperature accuracy | (Difference between the displayed temperature and temperature in the cell) 20°C to 40°C ±0.5°C ±1°C when the set temperature is outside the above range |
| Temperature setting difference | (Difference between the set temperature and displayed temperature) 20°C to 40°C ±0.3°C ±0.5°C when the set temperature is outside the above range |
Accessories
ETCS-761/762 Peltier thermostatted single position cell holder (water-cooled)
These cell holders use the Peltier effect to precisely maintain the sample temperature. The cell holders include a stirrer for uniform cell temperature. The ETCS-761 and ETCS-762 cell holders are water cooled Peltier accessories for a greater temperature range.
OPS-512 Optional temperature sensor for EHCS-760
OPS-512 OP sensor for direct temperature measurement in the sample cell for both the ETCS-761 or ETCS-762.
Specifications
10-mm rectangular cell (sample/reference – ETCS-762)
20°C to 40°C ± 0.5°C
± 1°C when the set temperature is outside the above range
20°C to 40°C ± 0.3°C
± 0.5°C when the set temperature is outside the above range
PWC-758 Automatic 8 x 8-position Peltier cell changer (water-cooled)
The PWC-758 is a multi-cell sample holder accessory for the simultaneous measurement of up to eight sample cells and 8 reference cells. The Peltier temperature control allows precise control of the sample temperature. A magnetic stirrer is used to maintain a uniform cell temperature for all 16 cell positions.
Cannot be used with the V-630 or V-730 (including bio versions)
Specifications
20°C to 40°C ±0.5°C
±1°C when the set temperature is outside the above range
PAC-743R Automatic 6/8-position Peltier Cell Changer (water-cooled and water-cooled with thermostatted reference)
The PAC-743 and PAC-743R automatic 6/8-Position Peltier cell changers are used for temperature controlled transmittance and absorbance measurements with multiple sample cells, with a variable temperature range from 0 to 100ºC.
Three cell block types are available: a 6-position 10 mm cell block, an 8-position micro cell block and an 8-position 5 mm cell block.
Using the 8-position micro cell block up to 8 samples can be simultaneously transferred directly from a 96 micro-titer plate (8x12) using an 8-way micro-pipette.
The PAC-743R also includes a temperature controlled reference position.
Cells for PAC-743/743R
5 mm rectangular
quartz cell
6-position cell block,
8 position cell block
8 position micro –cell block
Micro 8-position micro-cell
Accessories
Precise analysis of micro-volume samples for biological samples
The SAH-769 One Drop is a dedicated accessory for the V-600 and V-700 Series UV-visible spectrophotometers for the measurement of micro-volume samples of protein and nucleic acid. A precise optical pathlength is maintained, assuring excellent measurement reproducibility. The sampling procedure is very quick (around 20 seconds per sample) and easy, by simply placing a droplet of a sample on the disk cell window. The minimum sample volume is 5 µl for the standard 1 mm pathlength disk cell, and 0.6 µL for the optional 0.2 mm pathlength cell. The shorter optical pathlength allows measurements of high concentration samples without dilution.
Rapid, but highly accurate measurement using a flexible V-700 spectrophotometer. Only 20 seconds per sample.
Quantitative analysis of a 0.6 µl sample of calf thymus DNA using a 0.2 mm pathlength disk cell.
Spectra of calf
thymus DNA solutions
Calibration curve of calf thymus DNA
The figure illustrates excellent linearity up to approx.
3000 ng/µL. This is equivalent to a linearity of up
to 35 Abs for a 10 mm pathlength cell.
EMC-709 Micro cell holder
The EMC-709 is a micro cell holder for micro volume cells (approx. 50 µL or smaller). For use with rectangular micro cells with a path length up to 10 mm and a width of 2 mm, minimum.
length
Thickness
(Optionally available)X
1103-0194A
1103-0201A
6708-J046A
5 µL Micro cell (Normal cell)
5 µL Micro cell (Single sided cell)
Spacer for 5 µL Micro cell
50 µL Micro cell
Light path length: 10 mm
Light path width: 2 mm
Sample Volume: 50 µL
P/N: 1103 – 0114A
(Two sides are black quartz)
5 µL Micro cell（Normal type）
Light path length: 1 mm
Light path width: 2 mm
Sample Volume: 5 µL
P/N: 1103 – 0194A
5 µL Micro cell（Single sided type）
Light path length: 1 mm
Light path width: 2 mm
Sample Volume: 5 µL
P/N: 1103 – 0201A
Spacer for 5 µL Micro cell
After inserting a 5 µL Micro
cell into the spacer, it is
placed into the sample holder.
P/N: 6708 – J046A
EMC-759 5 µL Ultra-micro cell holder
The EMC-759 micro cell holder for micro volume cells (approx. 5 µL) can be used for minimal volume samples. Rectangular micro cells with a path length of 1 mm and a width of 2 mm are specified for use with this cell holder, using a lens to focus the light beam into the cell for precise low-volume measurements.
length
Thickness
(Available as option)
1103-0201A
5 µL Micro cell (Single sided cell)
Due to the optical path the EMC-759 is designed only for use with the V-630 and V-730 UV-Vis instruments (incl. Bio models) For other V-series instruments, please select the EMC-709 cell holder.
5 µL Micro cell (Normal type)
Light path length: 1 mm
Light path width: 2 mm
Sample Volume: 5 µL
P/N: 1103 – 0194A
5 µL Micro cell (Single sided type)
Light path length: 1 mm
Light path width: 2 mm
Sample Volume: 5 µL
P/N：1103 – 0201A
5 µL micro cell (stepped type)
This micro-volume cell (5 µL volume) is designed so that a pipette tip can reach the cell bottom and sample recovery can be easily performed. The dedicated cell holder, model EMC-759, is required to use this cell and the light beam is condensed using a lens integrated into the cell holder. Optimum measurements are obtained by reducing the source beam energy losses due to diffraction.
TCH-703 8-position micro turret cell holder
Minimum sample volume: 4 µL
The TCH-703 turret cell holder is designed for use only with the V-630/V-730 (incl. Bio models). The turret cell holder contains a ‘turret’ with 8 micro cells of 1 mm pathlength with a minimum sample volume of 4 µL each.
The cell position is manually selected with a guaranteed path length for reproducible results.
An optimized optical system reduces source beam energy losses due to diffraction of the incident beam. This optical system provides reproducible measurement of dilute and micro samples.
The turret can be removed for easy cleaning and loading of samples.
Cuvettes & Standards
| Part Number | Description |
|---|---|
| 1103-0181 | Rectangular glass cell 10 x 1 Special optical glass with lid 320 -2500 nm |
| 1103-0182 | Rectangular glass cell 10 x 2 Special optical glass with lid 320 -2500 nm |
| 1103-0105 | Rectangular glass cell 10 x 5 Special optical glass with lid 320 -2500 nm |
| 1103-0104 | Rectangular glass cell 10 x 10 Special optical glass with lid 320 -2500 nm |
| 1103-0103 | Rectangular glass cell 10 x 20 Special optical glass with lid 320 -2500 nm |
| 1103-0102 | Rectangular glass cell 10 x 50 Special optical glass with lid 320 -2500 nm |
| 1103-0101 | Rectangular glass cell 10 x 100 Special optical glass with lid 320 -2500 nm |
| Part Number | Description |
|---|---|
| 1103-0172 | Rectangular quartz cell 10 x 1 Spectrosil Quartz with lid 190 -2700 nm |
| 1103-0173 | Rectangular quartz cell 10 x 2 Spectrosil Quartz with lid 190 -2700 nm |
| 1103-0009 | Rectangular quartz cell 10 x 5 Spectrosil Quartz with lid 190 -2700 nm |
| 1103-0019 | Rectangular quartz cell 10 x 10 Spectrosil Quartz with lid 190 -2700 nm |
| 1103-0003 | Rectangular quartz cell 10 x 20 Spectrosil Quartz with lid 190 -2700 nm |
| 1103-0035 | Rectangular quartz cell 10 x 50 Spectrosil Quartz with lid 190 -2700 nm |
| 1103-0042 | Rectangular quartz cell 10 x 100 Spectrosil Quartz with lid 190 -2700 nm |
| Part Number | Description |
|---|---|
| 1103-0025 | Micro rectangular quartz cell 2 x 5 Spectrosil Quartz with lid 190 -2700 nm |
| 1103-0037 | Micro rectangular quartz cell 2 x 10 Spectrosil Quartz with lid 190 -2700 nm |
| 1103-0030 | Semi-micro rectangular quartz cell 4 x 10 Spectrosil Quartz with lid 190 -2700 nm |
| 1103-0032 | Micro rectangular quartz black cell 2 x 10 Spectrosil Quartz with lid 190 -2700 nm |
| 1103-0043 | Semi-micro rectangular quartz black cell 4 x 10 Spectrosil Quartz with lid 190 -2700 nm |
| 1103-W013 | Micro rectangular glass cell 2 x 5 Special optical glass with lid 320 -2500 nm |
| 1106-0015 | Micro short rectangular quartz black cell 2 x 10 Spectrosil Quartz with lid 190 -2700 nm |
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