Skip to content
Bio-Strategy Australia 1800 00 84 53 | Bio-Strategy New Zealand 0800 34 24 66

Remote Fluorescent Measurement using FP-8500 Fluorometer with Optical Fiber

Introduction

fluorometer with optical fiber
FP-8500 Spectrofluorometer

A fluorometer with optical fiber can be used for a variety of measurements, such as when the sample is larger than the sample chamber, to follow an in-situ reaction, or for measurement in hazardous environments, such as high/low temperature and high pressure. The OBF-832 optical fiber interface is used to connect an optical fiber for remote sampling of fluorescence materials with an FP-8000 Series fluorescence spectrophotometer. By using an optical fiber, measurement can be made by holding the probe close to or immersed in the sample.

In this application note, a comparison was made by measuring samples using an FP-8500 fluorometer with optical fiber and an FLH-809 film holder. To check for spectral accuracy the fiber probe was corrected for quantum efficiency using a calibrated light source with a validated spectral emission.

Experimental

Measurement System and Condition

FP-8500 Fluorescence spectrophotometer
OBF-832 Optical fiber unit
Calibration ESC-842 Calibration light source / standard white reference plate
Samples Red and Yellow reference cards

System Configuration

Measurement of the Calibrated Light Source under Dark Room Condition

Figure 1. Measurement system

Measurement Condition

Measurement Mode Fluorescence
Excitation Bandwidth 10 nm
Emission Bandwidth 10 nm
Excitation Wavelength 310 nm
Measurement Range 400 - 800 nm
Scan Speed 100 nm/min
Data Interval 0.5 nm
Response 1 sec
PMT Voltage 350 V (yellow card), 550 V (red card)

Keywords

Spectrofluorometer, Optical fiber, Fluorescence spectrum, fluorescence spectrophotometer

Result

Figures 2 and 3 show the measurement results. The profiles of the spectra of each colored sample measured using both the optical fiber and FLH-809 film holder were consistent with each other.

These results demonstrate that the spectral accuracy obtained using both the fiber probe and film holder yield comparable data.

Figure 2. Normalized fluorescence spectra of the yellow card

 

Figure 3. Normalized fluorescence spectra of the red card

Required Products and Software

  • FP-8300/8500/8600/8700 Spectrofluorometer
  • OBF-832 Optical Fiber Unit
  • ESC-842 Calibrated WI Light Source

Australia

Learn more ›

New Zealand

Learn more ›

Australia Freephone

1800 00 84 53

sales.au@bio-strategy.com

 

New Zealand Freephone

0800 34 24 66

sales.nz@bio-strategy.com

 

Bio-Strategy PTY Limited

MELBOURNE (HQ)

8 – 10 Trade Park Drive

Tullamarine, VIC 3043

Australia

 

BRISBANE

2/7 Miller Street

Murarrie, QLD 4172

Australia

 

Bio-Strategy Limited

33 Westpoint Drive

Hobsonville

Auckland 0618

New Zealand

Advanced Analytical Solutions For Your Science

Electromag-01

Copyright © 2023 Bio-Strategy Limited. Terms & Conditions

Scroll To Top