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Fluorescence Spectrometers

FP8550PC-480x211

Designed with the latest technology, the JASCO FP-8050 Series fluorescence spectrofluorometers incorporate the highest sensitivity, fastest spectral scanning capability and excellent analysis-oriented functionality offering integrated solutions for advanced materials research and biochemical analysis applications.

FP-8050 Series Models

Spectrofluorometers and accessories to meet all your needs of measuring for a wide range of applications from biological to advanced materials.
FP-8250-480x280

FP-8250 Spectrofluorometer

The FP-8250 is a simple and sensitive system which readily accommodates routine measurements and accessories, such as spectral scanning, quantitation, and temperature control.

FP-8350-480x280

The FP-8350 Spectrofluorometer is a powerful combination of performance, sensitivity and flexibility for biological, environmental and materials analysis.

FP-8550-480x267

The FP-8550 spectrofluorometer is a sophisticated optical system offering the ultimate in sensitivity, spectral accuracy, and flexibility for the most challenging materials and biological samples.

FP-8650-480x267

The FP-8650 is uniquely optimized for NIR applications with extended wavelength measurement to 1010 nm.

FP-8050 Series Features

The FP-8050 Series of spectrofluorometers includes four different instruments that provide solutions for the broadest range of applications including QC, biomolecular structural studies, environmental monitoring and advanced materials science. The FP-8050 Series has many flexible options for academic or industrial research, teaching, or use in quality control labs. Users can have the greatest confidence in their measurements, with an optical bench specifically designed for the highest sensitivity, widest dynamic range, and exceptional spectral purity with automatic cut-off filters to exclude higher order diffraction.

The FP-8050 Series combines a compact design with the largest range of accessories together with Spectra Manager™ Suite, a comprehensive data platform that gives you complete control over measurement, analysis and data archiving. In addition to the standard analysis programs, JASCO has developed many different applications software for dedicated sample measurement.

Advanced Features of the FP-8050 Series

  • High-throughput optical system
  • Highest S/N performance
  • Wide dynamic range (up to 7 orders of magnitude*)
  • Auto Gain and Auto Sensitivity Control System
  • Automatic cut-off filters for higher-order diffraction
  • Advanced digital signal processing
  • High speed scanning
  • Spectral bandwidth down to 1 nm

*Linearity for the FP-8550

Extensive Breadth of Features and Accessories

  • Compact benchtop footprint
  • Precision temperature control accessories for liquids and solids
  • Polarizers allow for automatic anisotropy measurements
  • Integrating spheres for Quantum Yield Determination
  • Microplate Reader for rapid sample throughput
  • Microsampling accessories for small volume samples
  • Stopped-flow systems for monitoring fast kinetics
  • Spectra Manager™ software for control and data analysis
  • Flexible design for expanding capabilities as needs evolve

Versatility for a Wide Range of Applications

  • Protein dynamics
  • Quantitative analysis
  • Cellular membrane studies
  • Enzyme kinetics
  • Water quality monitoring
  • Quantum dot and probe design
  • Carbon nanostructures and 2D materials
  • Fluorescent tracking materials
  • Short lifetime phosphorescence and PHOLEDs

Versatility for a Wide Range of Applications

Fluorescence applications including: Protein dynamics, Quantitative analysis, Cellular membrane studies, Enzyme kinetics, Carbon nanostructures, Characterization of fluorescent probes, Short life-time phosphorescence measurements, Thermal stability measurements, High Temperature and Up-Conversion.

Materials & Analytics Applications

Evaluation of FRET as a Sensitive Biosensor for Explosives

Fluorescence resonance energy transfer (FRET) is a mechanism governing the energy transfer between two neighboring molecules.

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Absolute Quantum Yields Using an Integrating Sphere

Different molecular and environmental conditions not only effect whether a molecule will fluoresce or not, but can also determine the intensity of the emitted fluorescence radiation.

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Internal Quantum Efficiency Measurement of Phosphor Powders

Some white LEDs consist of two types of materials: a luminescent diode that emits a blue light in the near-UV region, and a phosphor that emits visible light from the absorption to the near-UV region. 

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Remote Fluorescent Measurement using FP-8500 Fluorometer with Optical Fiber

A fluorometer with optical fiber can be used for a variety of measurements, such as when the sample is larger than the sample chamber, to follow an in-situ reaction, or for measurement in hazardous environments, such as high/low temperature and high pressure.

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Quantum Yield Measurement of the Up-Conversion Phosphors

Quantum yield is very useful in determining the efficiency of multi-step up-conversion excitation process that results in fluorescence at shorter wavelengths than the excitation light. Molecules can be excited at longer wavelengths where light scattering and absorption do not occur.

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Life Sciences Applications

Determination of the Relative Quantum Yield of Rhodamine-B

Different molecular and environmental conditions not only effect whether a molecule will fluoresce or not, but can also determine the intensity of the emitted fluorescence radiation.

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Micro-volume Analysis of a λ DNA Using a One Drop Accessory

For many biological samples, it is much more convenient, cost effective, and efficient to use microvolumes for structural and quantitative studies.

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Near-Infrared Phosphor Measurement

Near-infrared fluorescence labels are currently attracting the attention of the scientific community due to their wide range of biological applications.

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Measurement of Trypsin Activity Using a Fluorescence Peptide Substrate

In biology, proteolytic reactions occur to regulate enzyme and protein activity for metabolic to signal processing functions. Proteases are responsible for breaking down biological molecules into smaller polypeptide chains through hydrolysis. 

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Food & Beverages Application

High-Speed Excitation Emission Matrix Measurement of Olive Oil

Excitation-Emission Matrix (EEM) is used with fluorescence spectroscopy for identification and characterization in mixture analysis. Recently, EEM has started to become an accepted method for determining the type, quality, and geographical origin of food products.

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Environmental Application

Component Analysis of an Excitation-Emission Matrix of Environmental Water Samples Using PARAFAC Analysis

Parallel Factor Analysis (PARAFAC) is a type of multivariable analysis, which can be used to extract the components from mixed 3D fluorescence data. JASCO has developed an advanced solution using PARAFAC, which can help to interpret the complicated data found in the EEM.

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FP-8050 Series Specifications

Model
FP-8250
FP-8350
FP-8550
FP-8650
Light Source
Continuous output Xe arc lamp with shielded lamp housing (150 W) Lifetime 3000 hours
Light Source (for validation)
Integrated, selectable low pressure mercury lamp
Photometric System
Ratio-photometer system using monochromatic light to monitor the intensity output of the Xe lamp
Monochromator
Holographic concave grating in modified Rowland mount
Wavelength Range (with Standard Detector)
Ex
Zero order, 200 - 750 nm
Zero order, 200 - 750 nm
Zero order, 200 - 850 nm
Zero order, 200 - 850 nm
Em
Zero order, 200 - 980nm
Wavelength Range (Optional)
200 - 900nm
200 - 1010nm
Automatic Cut Filters for High Order Diffraction
Option
Standard
Sensitivity *1 (RMS)
Base *2
4,500 : 1
8,000 : 1
8,500 : 1
3,500 : 1
Band Width
Ex
2.5, 5, 10, 20 nm
1, 2.5, 5, 10, 20 nm
1, 2.5, 5, 10, 20, L5, L10 nm
1, 2.5, 5, 10, 20, L5, L10 nm
Em
2.5, 10, 20, 40, L10, L20 nm
Wavelength Accuracy
Ex
±2.0 nm
±1.5 nm
±1.0 nm
±1.0 nm
Em
±2.0 nm
Wavelength Scan Speed
Ex
20, 50, 100, 200, 500, 1,000, 2,000, 5,000,
10,000, 20,000 nm/min
10, 20, 50, 100, 200, 500, 1,000, 2,000, 5,000, 10,000, 20,000,
60,000 nm/min
10, 20, 50, 100, 200, 500, 1,000, 2,000, 5,000, 10,000, 20,000, 60,000 nm/min
Em
20, 50, 100, 200, 500, 1,000, 2,000, 5,000, 10,000, 20,000, 60,000, 120,000 nm/min
Detector
Ex: Silicon photodiode, Em: PMT
Auto Gain
Standard
Shutter Function
Standard (Automatic control)
Sample Illuminating System
Horizontal illumination
Sample Compartment
10 mm rectangular cell holder, nitrogen purgeable
Accessory Recognition
Standard
Start Button
Standard
Analog Output
Standard
Instrument Communication
USB 2.0
Control and Data Processing
Spectra Manager™/CFR, iRM
Spectra Manager™/CFR
Spectral Correction
Option
Standard
(Spectral correction using a Rhodamine B ethylene glycol solution is standard; other jigs for spectral correction are available separately as options.)
Dimensions
520(W) × 545(D) × 270(H) mm
570(W) × 545(D) × 270(H) mm
Weight
36 kg (79 lbs)
39 kg (86 lbs)
Power Requirement
270VA
Installation Environment
Temperature: 15 to 35°C, Humidity: Less than 85%

*1 : Minimum Signal-to-Noise ratio of Raman band of water, excitation 350 nm, band width Ex 5 nm Em 5 nm (FP-8650: Ex 5 nm Em 10 nm), response 2 seconds.
*2 : Noise is measured on the baseline.

Software Specification

A wide range of software applications and accessories can be used with Spectra Manager™ Suite.

Model
FP-8250
FP-8350
FP-8550
FP-8650
Data Station Type
Spectra Manager™/Spectra Manager™ CFR (Microsoft Windows® 10 Professional or Enterprise)
iRM Type
Handheld Intelligent Remote Module iRM-900
Not Used
Measurement Programs
Spectra Manager™
Spectra measurement,

Quantitative measurement,

Fixed wavelength measurement,

Time course measurement,

3D Spectra measurement,

Absorbance measurement

Spectra measurement,

Quantitative measurement,

Fixed wavelength measurement,

Time course measurement,

3D Spectra measurement,

Absorbance measurement,

Phosphorescence measurement

Spectra Manager™ CFR *4
iRM-900 *5
Spectra measurement, Quantitative measurement, Fixed wavelength measurement, Time course measurement, 3D Spectra measurement, Absorbance measurement
Not Used
Spectra Correction Program
Standard *6
Standard
Daily Check
Standard
Instrument Validation
Program and Hg lamp (Standard), Accessories (option)
Program and Hg lamp (Standard), Accessories (Standard)
Self Diagnosis
Standard
IQ Accessory Recognition and IQ Start
Standard

 

*4 : Spectra Manager™ CFR is the 21 CFR Part 11 compliant software package.
*5 : The iRM-900 handheld control module can be used with any PictBridge compatible printer.
*6 : Optional components are necessary.
Specifications are subject to change without notice.

Australia

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New Zealand

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Australia Freephone

1800 00 84 53

sales.au@bio-strategy.com

 

New Zealand Freephone

0800 34 24 66

sales.nz@bio-strategy.com

 

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Australia

 

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Australia

 

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New Zealand

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