Fluorescence Spectrometers

Designed with the latest technology, the JASCO FP-8050 Series fluorescence spectrofluorometers incorporate the highest sensitivity, fastest spectral scanning capability and excellent analysis-oriented functionality offering integrated solutions for advanced materials research and biochemical analysis applications.

FP-8050 Series Models

Spectrofluorometers and accessories to meet all your needs of measuring for a wide range of applications from biological to advanced materials.

FP-8250 Spectrofluorometer

The FP-8250 is a simple and sensitive system which readily accommodates routine measurements and accessories, such as spectral scanning, quantitation, and temperature control.

FP-8350 Spectrofluorometer

The FP-8350 Spectrofluorometer is a powerful combination of performance, sensitivity and flexibility for biological, environmental and materials analysis.

FP-8550 Spectrofluorometer

The FP-8550 spectrofluorometer is a sophisticated optical system offering the ultimate in sensitivity, spectral accuracy, and flexibility for the most challenging materials and biological samples.

FP-8650 NIR Spectrofluorometer

The FP-8650 is uniquely optimized for NIR applications with extended wavelength measurement to 1010 nm.

FP-8050 Series Features

The FP-8050 Series of spectrofluorometers includes four different instruments that provide solutions for the broadest range of applications including QC, biomolecular structural studies, environmental monitoring and advanced materials science. The FP-8050 Series has many flexible options for academic or industrial research, teaching, or use in quality control labs. Users can have the greatest confidence in their measurements, with an optical bench specifically designed for the highest sensitivity, widest dynamic range, and exceptional spectral purity with automatic cut-off filters to exclude higher order diffraction.

The FP-8050 Series combines a compact design with the largest range of accessories together with Spectra Manager™ Suite, a comprehensive data platform that gives you complete control over measurement, analysis and data archiving. In addition to the standard analysis programs, JASCO has developed many different applications software for dedicated sample measurement.

Advanced Features of the FP-8050 Series

High-throughput optical system
Highest S/N performance
Wide dynamic range (up to 7 orders of magnitude*)
Auto Gain and Auto Sensitivity Control System
Automatic cut-off filters for higher-order diffraction
Advanced digital signal processing
High speed scanning
Spectral bandwidth down to 1 nm

*Linearity for the FP-8550

Extensive Breadth of Features and Accessories

Compact benchtop footprint
Precision temperature control accessories for liquids and solids
Polarizers allow for automatic anisotropy measurements
Integrating spheres for Quantum Yield Determination
Microplate Reader for rapid sample throughput
Microsampling accessories for small volume samples
Stopped-flow systems for monitoring fast kinetics
Spectra Manager™ software for control and data analysis
Flexible design for expanding capabilities as needs evolve

Versatility for a Wide Range of Applications

Protein dynamics
Quantitative analysis
Cellular membrane studies
Enzyme kinetics
Water quality monitoring
Quantum dot and probe design
Carbon nanostructures and 2D materials
Fluorescent tracking materials
Short lifetime phosphorescence and PHOLEDs

Please download the JASCO FP-8050 Series brochure

Versatility for a Wide Range of Applications

Fluorescence applications including: Protein dynamics, Quantitative analysis, Cellular membrane studies, Enzyme kinetics, Carbon nanostructures, Characterization of fluorescent probes, Short life-time phosphorescence measurements, Thermal stability measurements, High Temperature and Up-Conversion.

Materials & Analytics Applications

Evaluation of FRET as a Sensitive Biosensor for Explosives

Fluorescence resonance energy transfer (FRET) is a mechanism governing the energy transfer between two neighboring molecules.

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Absolute Quantum Yields Using an Integrating Sphere

Different molecular and environmental conditions not only effect whether a molecule will fluoresce or not, but can also determine the intensity of the emitted fluorescence radiation.

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Internal Quantum Efficiency Measurement of Phosphor Powders

Some white LEDs consist of two types of materials: a luminescent diode that emits a blue light in the near-UV region, and a phosphor that emits visible light from the absorption to the near-UV region.

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Remote Fluorescent Measurement using FP-8500 Fluorometer with Optical Fiber

A fluorometer with optical fiber can be used for a variety of measurements, such as when the sample is larger than the sample chamber, to follow an in-situ reaction, or for measurement in hazardous environments, such as high/low temperature and high pressure.

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Quantum Yield Measurement of the Up-Conversion Phosphors

Quantum yield is very useful in determining the efficiency of multi-step up-conversion excitation process that results in fluorescence at shorter wavelengths than the excitation light. Molecules can be excited at longer wavelengths where light scattering and absorption do not occur.

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Life Sciences Applications

Determination of the Relative Quantum Yield of Rhodamine-B

Different molecular and environmental conditions not only effect whether a molecule will fluoresce or not, but can also determine the intensity of the emitted fluorescence radiation.

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Micro-volume Analysis of a λ DNA Using a One Drop Accessory

For many biological samples, it is much more convenient, cost effective, and efficient to use microvolumes for structural and quantitative studies.

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Near-Infrared Phosphor Measurement

Near-infrared fluorescence labels are currently attracting the attention of the scientific community due to their wide range of biological applications.

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Measurement of Trypsin Activity Using a Fluorescence Peptide Substrate

In biology, proteolytic reactions occur to regulate enzyme and protein activity for metabolic to signal processing functions. Proteases are responsible for breaking down biological molecules into smaller polypeptide chains through hydrolysis.

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Please download optional Plate Reader with full scanning capability and temperature control now available.

Food & Beverages Application

High-Speed Excitation Emission Matrix Measurement of Olive Oil

Excitation-Emission Matrix (EEM) is used with fluorescence spectroscopy for identification and characterization in mixture analysis. Recently, EEM has started to become an accepted method for determining the type, quality, and geographical origin of food products.

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Environmental Application

Component Analysis of an Excitation-Emission Matrix of Environmental Water Samples Using PARAFAC Analysis

Parallel Factor Analysis (PARAFAC) is a type of multivariable analysis, which can be used to extract the components from mixed 3D fluorescence data. JASCO has developed an advanced solution using PARAFAC, which can help to interpret the complicated data found in the EEM.

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FP-8050 Series Specifications

Model	FP-8250	FP-8350	FP-8550	FP-8650
Light Source	Continuous output Xe arc lamp with shielded lamp housing (150 W) Lifetime 3000 hours
Light Source (for validation)	Integrated, selectable low pressure mercury lamp
Photometric System	Ratio-photometer system using monochromatic light to monitor the intensity output of the Xe lamp
Monochromator	Holographic concave grating in modified Rowland mount
Wavelength Range (with Standard Detector)
Ex	Zero order, 200 - 750 nm	Zero order, 200 - 750 nm	Zero order, 200 - 850 nm	Zero order, 200 - 850 nm
Em	Zero order, 200 - 750 nm	Zero order, 200 - 750 nm	Zero order, 200 - 850 nm	Zero order, 200 - 980nm
Wavelength Range (Optional)		200 - 900nm		200 - 1010nm
Automatic Cut Filters for High Order Diffraction	Option	Standard
*Sensitivity ^1(RMS)**
Base ^*2	4,500 : 1	8,000 : 1	8,500 : 1	3,500 : 1
Band Width
Ex	2.5, 5, 10, 20 nm	1, 2.5, 5, 10, 20 nm	1, 2.5, 5, 10, 20, L5, L10 nm	1, 2.5, 5, 10, 20, L5, L10 nm
Em	2.5, 5, 10, 20 nm	1, 2.5, 5, 10, 20 nm	1, 2.5, 5, 10, 20, L5, L10 nm	2.5, 10, 20, 40, L10, L20 nm
Wavelength Accuracy
Ex	±2.0 nm	±1.5 nm	±1.0 nm	±1.0 nm
Em	±2.0 nm	±1.5 nm	±1.0 nm	±2.0 nm
Wavelength Scan Speed
Ex	20, 50, 100, 200, 500, 1,000, 2,000, 5,000, 10,000, 20,000 nm/min		10, 20, 50, 100, 200, 500, 1,000, 2,000, 5,000, 10,000, 20,000, 60,000 nm/min	10, 20, 50, 100, 200, 500, 1,000, 2,000, 5,000, 10,000, 20,000, 60,000 nm/min
Em				20, 50, 100, 200, 500, 1,000, 2,000, 5,000, 10,000, 20,000, 60,000, 120,000 nm/min
Detector	Ex: Silicon photodiode, Em: PMT
Auto Gain	Standard
Shutter Function	Standard (Automatic control)
Sample Illuminating System	Horizontal illumination
Sample Compartment	10 mm rectangular cell holder, nitrogen purgeable
Accessory Recognition	Standard
Start Button	Standard
Analog Output	Standard
Instrument Communication	USB 2.0
Control and Data Processing	Spectra Manager™/CFR, iRM		Spectra Manager™/CFR
Spectral Correction	Option		Standard (Spectral correction using a Rhodamine B ethylene glycol solution is standard; other jigs for spectral correction are available separately as options.)
Dimensions	520(W) × 545(D) × 270(H) mm		570(W) × 545(D) × 270(H) mm
Weight	36 kg (79 lbs)		39 kg (86 lbs)
Power Requirement	270VA
Installation Environment	Temperature: 15 to 35°C, Humidity: Less than 85%

^*1 : Minimum Signal-to-Noise ratio of Raman band of water, excitation 350 nm, band width Ex 5 nm Em 5 nm (FP-8650: Ex 5 nm Em 10 nm), response 2 seconds.
^*2 : Noise is measured on the baseline.

Software Specification

A wide range of software applications and accessories can be used with Spectra Manager™ Suite.

Model	FP-8250	FP-8350	FP-8550
Data Station Type	Spectra Manager™/Spectra Manager™ CFR (Microsoft Windows® 10 Professional or Enterprise)
iRM Type	Handheld Intelligent Remote Module iRM-900		Not Used
Measurement Programs
Spectra Manager™	Spectra measurement, Quantitative measurement, Fixed wavelength measurement, Time course measurement, 3D Spectra measurement, Absorbance measurement	Spectra measurement, Quantitative measurement, Fixed wavelength measurement, Time course measurement, 3D Spectra measurement, Absorbance measurement, Phosphorescence measurement
Spectra Manager™ CFR ^*4
iRM-900 ^*5	Spectra measurement, Quantitative measurement, Fixed wavelength measurement, Time course measurement, 3D Spectra measurement, Absorbance measurement		Not Used
Spectra Correction Program	Standard ^*6	Standard
Daily Check	Standard
Instrument Validation	Program and Hg lamp (Standard), Accessories (option)		Program and Hg lamp (Standard), Accessories (Standard)
Self Diagnosis	Standard
IQ Accessory Recognition and IQ Start	Standard

^*4 : Spectra Manager™ CFR is the 21 CFR Part 11 compliant software package.
^*5 : The iRM-900 handheld control module can be used with any PictBridge compatible printer.
^*6 : Optional components are necessary.
Specifications are subject to change without notice.

Australia

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New Zealand

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Australia Freephone

1800 00 84 53

sales.au@bio-strategy.com

New Zealand Freephone

0800 34 24 66

sales.nz@bio-strategy.com

Bio-Strategy PTY Limited

MELBOURNE (HQ)

8 – 10 Trade Park Drive

Tullamarine, VIC 3043

Australia

BRISBANE

2/7 Miller Street

Murarrie, QLD 4172

Australia

Bio-Strategy Limited

33 Westpoint Drive

Hobsonville

Auckland 0618

New Zealand

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