Analytical SFC - SF-4000

With up to two modifier pumps and six solvent selection units, modifier selection is very flexible and enables rapid screening of a range of columns using different solvent conditions completely automatically. The SFC-4000 has an option for up to three column ovens each with ten full sized columns with separate temperature control, great for combining chiral and achiral SFC in a single system. Take advantage of the PDA detector combined with the CD-4095 chiral detector to work up fast analytical methods for direct scale-up to semi-prep and prep separations.

Features

The SFC-4000 Analytical SFC system comprises:

PU-4380 CO2 pump

The PU-4380-CO₂ peltier temperature stabilized pump ensures the optimum conditions for pumping liquid CO₂, there is no requirement for complicated pretreatment of CO2 and no need for a chiller for cooling the pumps heads. Simply supply low cost beverage grade CO₂.Automated stop valves control the input and output of CO₂.

Modifier pumps

Choose either one or two PU-4180 modifier pumps to add to the system with optional 6 or 12 solvent selection for method development.

Column temperature control

Peltier stabilized CO-4060 column oven for accurate control (from below the CO₂ critical point up to 80 degC) with large space to accommodate six 4.6mm or 10mm columns (up to 300mm length), with optional column switching. Use 2 ovens for different temperature zones and for multiple column options for chiral and achiral screening. CO-4065 with internal column switching for analytical and prep columns up to 3cm ID.

Back pressure regulator

The BP-4380 remains the best solution for maintaining supercritical conditions with pressure fluctuations as low as 1.5 psi peak to peak. Dynamic control enables modifier gradients without changes to back pressure. Response is fast and accurate ensuring stable baselines even for trace level analysis.

Detection

The SFC-4000 system offers the widest selection of detectors available for Analytical SFC.

UV-Visible

The SF-4000 system includes the UV-4070/75 for dual wavelength 190 to 600nm (or 900nm).

Photometric Diode Array PDA

The SFC-4000 system is equipped with MD-4010, a 1024-element diode array for high sensitivity, trace analysis and component identification.

Chiral

The CD-4095 circular dichroism detector is the only chiral detector compatible with SFC and provides positive confirmation of enantiomeric separation. It can also be used to drive chiral directed prep SFC.

ELSD and FID

ELSD and FID detection can be used by splitting a portion of flow to inlet of the detector; these detectors are great for material that have no absorbing chromophore such as in petrochemical or pharmaceutical analysis.

Mass Spectrometry Detection with SFC

The SF-4000 analytical can be used with virtually any mass spectrometer working with ESI, APCI or APPI interface.

Instrument Control and Data Acquisition

The SFC-4000 system is completely controlled using the ChromNAV data system - offering simple control for routine analytical separation through to complex method development with a range of enhanced reporting capabilities. Full GxP compliance and 21 CFR part 11.

Details

Applications for the JASCO SFC-4000 Analytical SFC System

Simple analytical

JASCO SFC-4000 Analytical SFC system operates with flow rates between 0.5 to 7mL/min (Liquid CO2).

Method development

With up to 2 modifier pumps and 6 or 10 solvent selection units, modifier selection is very flexible and enables rapid screening of a range of columns using different solvent conditions completely automatically. The SFC-4000 has an option for up to 3 column ovens each with up to 10 full sized columns with separate temperature control, great for combining chiral and achiral SFC in a single system.

Take advantage of the MD-4010 PDA detector combined with the CD-4095 chiral detector to work up fast analytical methods for direct scale-up to semi-prep and prep separations.

Small Scale Prep

Using 4.6mm columns with a choice of either 6 or 8 way fraction valves or the new open bed fraction collector with MCS 6 micro-cyclone collection system (patented), the SFC-4000 system is the ideal choice for small scale (sub mg) preparation using analytical SFC.