The LC-4000 Series includes three PDA detectors. The MD-4017 is a useful replacement for a single wavelength detector, the MD-4015 is the workhorse for UV- visible detection in the range 200 to 600nm and the MD-4010 is designed for high sensitivity and high spectral and time resolution.

The MD-4010 PDA detector offers the highest detection sensitivity, equivalent to a single wavelength detector. Making use of a 1024 element photometric diode array, the measurement wavelength range is from 190nm to 900nm with a MD-4010 PDA resolution of 1nm and fast spectrum acquisition rate of up to 100 spectra/sec. When used with the new temperature controlled SP type semi-micro flow cell the MD-4010 is well suited to the narrower peaks typically found with UHPLC or RHPLC separations. The MD-4010 is fitted with an internal mercury lamp for automatic wavelength calibration.

The MD-4015 PDA detector is the routine detector for conventional HPLC, RHPLC and UHPLC. The sensitivity is almost equivalent to that of the MD-4010 but only covers the wavelength range from 200nm to 600nm using a 512 element PDA for 1nm resolution and with high speed 100Hz spectrum acquisition.

Using the advanced Czerny- Turnermonochromator design with optimization of the optical system, baseline change due to refractive index is minimized. Baseline fluctuation from changes in environmental temperature is reduced with a temperature - controlled cell, baseline drift caused by temperature fluctuation in the light source is also reduced with a temperature controlled light source. The result is excellent stability with UV-4070 UV Visible UV spectra auto-scan Automatic spectral scannning can be controlled using ChromNAV, when a peak exceeds a set threshold a scan is executed automatically. 0.24 220000 200000 the noise level +/- 0.2x10 -5 AU and drift 1x10 -4 AU/h.

Both detectors offer simultaneous dual-wavelength data acquisition and spectral scanning. User maintenance can be done without removing the module from the system. Front-panel access is used for light source replacement and automatic wavelength calibration can be carried out using a mercury lamp. Both detectors include high speed data acquisition at up to 100Hz and can be used for conventional HPLC with the standard flow cell and for UHPLC/RHPLC when used with the SP type semi-micro cell.

The FP-4020 fluorescence detector has been designed for the pursuit of extreme sensitivity. Re-designing the optics and electronics from the ground up, as well as removing the effects of ambient temperature with a new temperature- regulated cell body has led to the highest sensitivity with stable detection. The new axially irradiated cell design enables the very highest sensitivity detection. These changes have resulted in a giant leap in sensitivity for the FP-4020 to achieve a signal noise ratio of 2300:1 or higher (based on the water Raman peak). The FP-4025 is an excellent routine fluorescence detector that has a slightly lower specification with a water Raman S/N of 1400:1 .

Both detectors can be used for simultaneous detection at two wavelength pairs; excitation and emission wavelengths can be changed during the acquisition using a time program and automatic spectra scanning can be triggered manually, by time or automatically by threshold. A built-in mercury lamp enables automatic wavelength correction and the front-access design allows easy replacement of the flow cell assembly and light source.

The Refractive Index detectors include the optical system mounted in a precisely temperature-regulated housing to eliminate the influence of changes in ambient temperature. The RI-4030 and 4035 detectors are fully stable just one hour after powering on. The RI-4030 can be used for both analytical and preparative scales. In preparative mode the maximum flow rate is 120 ml/min. The RI-4035 is a dedicated semi-micro RI detector with a micro-volume flow cell designed for use with semi-micro GPC analysis using GPC columns with a fine pore size.

The 100Hz data acquisition rate makes the RI-4035 suitable for RHPLC and UHPLC separations. The LED light source in both detectors has a long life time (over 10,000 hours) for low maintenance operation. Both the RI-4030 and RI-4035 detectors can be fitted with an optional pressure release safety-valve to prevent damage to the flow cell in the event blockage.

The CD-4095 is a unique HPLC detector for measuring circular dichroism developed from the market leading J-Series circular dichroism spectropolarimeter. Optically active compounds with a chromophore close to a chiral center may absorb circularly polarized light, which can be detected with excellent sensitivity and selectivity. When chiral compounds are measured using a UV-Visible detector, the d- and l- enantiomers cannot be distinguished even when separated by a chiral column.

The CD-4095 circular dichroism detector makes it possible to positively distinguish between the d- and l- enantiomers by measuring the positive and negative peaks. It is also possible to quantify the d- and l-forms even when they are not separated, a calibration curve based on the g-factor can be used to determine the enantiomeric ratio. The ChromNAV CDS can be used to simultaneously measure the CD, g-factor data and UV-visible signal. The CD spectra of eluted compounds can be measured by triggering scanning manually, by timed program or automatically by threshold.

The OR-4090 Chiral detector measures the angle of rotation of plane polarized light caused by optical active isomers and is useful for chiral compounds with no absorption. The OR-4090 uses a high intensity Hg-Xe arc lamp (150W) providing a strong output in the UV/Visible region where the largest optical rotations offer the highest sensitivity. The wide wavelength range from 350-900nm also allows measurement of a wide variety of samples. One of the key benefits of optical rotation detection is that it is unaffected by changes in solvent and can be used with gradient elution, unlike an RI detector.